Journal: Biomaterials Research

Article Title: Versatile Nanotherapeutics for Enhancing Sonodynamic Therapy/Chemotherapy of Thyroid Cancer through Remodeling Tumor Microenvironment and Synergistic Reactive Oxygen Species Augment

doi: 10.34133/bmr.0338

Figure Lengend Snippet: FL@M enhances oxidative stress and induces apoptosis. (A) ROS production in 8505C cells after different treatments was detected by DCFH-DA. (B) Quantification of intracellular fluorescence intensity of DCFH-DA shown in (A). (C) Lipid oxidation levels in 8505C cells after different treatments were detected by BODIPY. (D) Quantification of intracellular fluorescence intensity of lipid oxidation level shown in (C). (E) The changes in the MMP of 8505C cells after different treatments were detected by JC-1. (F) Quantification of intracellular fluorescence intensity of MMP shown in (E). (G) Flow cytometry was used to assess ROS production after different treatments detected by DCFH-DA. (H) Flow cytometry was used to assess lipid oxidation levels after different treatments detected by BODIPY. (I) Flow cytometry was used to assess MMP after different treatments detected by JC-1. (J) Apoptosis of 8505C cells was assessed by flow cytometry with Annexin V-PE/7AAD staining. (K) Western blotting analysis of GPX4, MMP2, MMP9, MMP1, and E-Cad protein in 8505C cells with various treatments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The primary antibodies used in this study were as follows: GPX4 (ab125066, Abcam), MMP2 (10373-2-AP, Proteintech), MMP9 (10375-2-AP, Proteintech), MMP1 (10371-2-AP, Proteintech), E-cadherin (20874-1-AP, Proteintech), HIF-1α (20960-1-AP, Proteintech), Galectin-3 (82024-1-RR, Proteintech), Vinculin (A2752, ABclonal), and ACTB (AC026, ABclonal).

Techniques: Fluorescence, Flow Cytometry, Staining, Western Blot

Journal: Cell Proliferation

Article Title: Soluble Sema4D From γδ T Cells Exerts Osteoblast Inhibition via Plexin‐B/ mTOR Signalling Contributing to Pathogenesis of Bisphosphonate‐Related Osteonecrosis of the Jaws

doi: 10.1111/cpr.70114

Figure Lengend Snippet: Autocrine MMP3 secretion induced mSema4D cleavage in γδ T cells. (A) KEGG analysis of DEGs in WT ZOL and TCRδ −/− ZOL mice was highly enriched in matrix metalloproteinase (MMP)‐related pathways. (B) The mRNA levels of Mmp3 and Adam17 in the extraction sockets of WT ZOL and TCRδ −/− ZOL mice. n = 4, * p < 0.05, ns indicates no significance. (C) The mRNA levels of Adam17 , Adam10 , Mmp2 , Mmp3 , Mmp9 and Mmp11 after γδ T cells were incubated with 15 μM ZOL for 3 and 6 days. Only the mRNA level of Mmp3 increased significantly and kept a high level after 6 days incubation. n = 6, * p < 0.05, ** p < 0.01. (D) The protein level of MMP3 was much higher than MMP2 and MMP9 in the γδ T cell supernatant measured by ELISA. n = 4, ** p < 0.01, ns indicates no significance. (E) The proportions of γδ T cells after treatment with GM6001 and TAPI‐2. GM6001 and TAPI‐2 are MMP inhibitors. T10 and T20 indicate 10 and 20 nM TAPI‐2, respectively; G50 and G100 indicate 50 and 100 nM GM6001, respectively. n = 6, ns indicates no significance. (F) The expression of Sema4D on γδ T cells after treatment with GM6001 and TAPI‐2. (G) Analysis of Sema4D expression in γδ T cells after treatment with GM6001 or TAPI‐2 on day 2 and 4. n = 6, * p < 0.05, ** p < 0.01. (H) The level of sSema4D in the supernatant of γδ T cells after treatment with GM6001 or TAPI‐2. n = 3, * p < 0.05, ** p < 0.01. (I) The level of sSema4D in the supernatant of γδ T cells after incubating with MMP2, MMP3 and MMP9 in synergy with GM6001. n = 4, * p < 0.05, ** p < 0.01. Data are mean ± SD.

Article Snippet: The kits of sSema4D (ELK5378, ELK Biotechnology), MMP2 (E‐EL‐M0780, Elabscience), MMP3 (E‐EL‐M0626) and MMP9 (E‐EL‐M3052) were used following the manufacturer's instructions.

Techniques: Extraction, Incubation, Enzyme-linked Immunosorbent Assay, Expressing